Scanning holographic microscopy with resolution exceeding the Rayleigh limit of the objective by superposition of off-axis holograms
Applied Optics, Vol. 46, Issue 6, pp. 993-1000 doi:10.1364/AO.46.000993
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- OCIS Codes:
- (090.0090) Holography : Holography
- (110.0180) Imaging systems : Microscopy
- (110.6880) Imaging systems : Three-dimensional image acquisition
- (180.0180) Microscopy : Microscopy
- (180.2520) Microscopy : Fluorescence microscopy
Microscopy
Citation
Guy Indebetouw, Yoshitaka Tada, Joseph Rosen, and Gary Brooker, "Scanning holographic microscopy with resolution exceeding the Rayleigh limit of the objective by superposition of off-axis holograms," Appl. Opt. 46, 993-1000 (2007)
http://www.opticsinfobase.org/abstract.cfm?URI=ao-46-6-993
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We present what we believe to be a new application of scanning holographic microscopy to superresolution. Spatial resolution exceeding the Rayleigh limit of the objective is obtained by digital coherent addition of the reconstructions of several off-axis Fresnel holograms. Superresolution by holographic superposition and synthetic aperture has a long history, which is briefly reviewed. The method is demonstrated experimentally by combining three off-axis holograms of fluorescent beads showing a transverse resolution gain of nearly a factor of 2.
© 2007 Optical Society of America
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History
Original Manuscript: August 30, 2006
Manuscript Accepted: October 16, 2006
Revised Manuscript: October 13, 2006
Published: February 2, 2007
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