Abstract

We demonstrate a new method of measuring quantitative phase in imaging of biological materials. This method, asynchronous digital holography, employs knowledge of a moving fringe created by acousto-optic modulators to execute phase-shifting interferometry using two near-simultaneous interferograms. The method can be used to obtain quantitative phase images of dynamic biological samples on millisecond time scales. We present results on a standard sample, and on live cell samples.

© 2007 Optical Society of America

» View Full Text: Acrobat PDF (1178 KB) 

History
Original Manuscript: January 16, 2007
Manuscript Accepted: March 10, 2007
Revised Manuscript: March 9, 2007
Published: March 19, 2007

References

1. P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, and T. Colomb, C. Depeursinge, "Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy," Opt. Lett. 30, 468 (2005). [CrossRef]
2. N. Lue, G. Popescu, T. Ikeda, R. R. Dasari, K. Badizadegan, and M. S. Feld, "Live cell refractometry using microfluidic devices," Opt. Lett. 31, 2759 (2006). [CrossRef]
3. T. Kreis, Holographic interferometry : principles and methods (Berlin: Akademie Verlag, 1996).
4. T. Zhang and I. Yamaguchi, "Three-dimensional microscopy with phase-shifting digital holography," Opt. Lett. 23, 1221 (1998).
5. E. Cuche, F. Bevilacqua, and C. Depeursinge, "Digital holography for quantitative phase-contrast imaging," Opt. Lett. 24, 291 (1999).
6. L. Xu, J. Miao, and A. Asundi, "Properties of digital holography based on in-line configuration," Opt. Eng. 39, 3214 (2000). [CrossRef]
7. T. Ikeda, G. Popescu, R. R. Dasari, and M. S. Feld, "Hilbert phase microscopy for investigating fast dynamics in transparent systems," Opt. Lett. 30, 1165 (2005). [CrossRef]
8. T. Kreis, "Digital Holographic Interference-Phase Measurement Using the Fourier-Transform Method," J. Opt. Soc. A 3, 847 (1986).
9. G. Popescu, T. Ikeda, and R. R. Dasari, "Diffraction phase microscopy for quantifying cell structure and dynamics," Opt. Lett. 31, 775 (2006). [CrossRef]
10. E. B. Li, J. Q. Yao, D. Y. Xu, J. T. Xi, and J. Chicharo, "Optical phase shifting with acousto-optic devices," Opt. Lett. 30, 189 (2005). [CrossRef]
11. U. Schnars. and W. Jueptner, Digital holography : digital hologram recording, numerical reconstruction, and related techniques (Berlin, Springer, 2005).
12. S. L. Hahn, Hilbert transforms in signal processing (Artech House, Boston, 1996).
13. D. C. Ghiglia and M. D. Pritt, Two-dimensional phase unwrapping : theory, algorithms, and software (New York, Wiley, 1998).

Citing Articles

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Advanced Search

Recent ToC Categories (Beta)