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Improving analysis from second-derivative uv-absorption spectrometry

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Abstract

Methods for improving the analytical results obtained from second-derivative spectrometry are presented. By using a combination of the first and second derivatives of the spectral data, a response varying linearly with concentration can be obtained at all wavelengths if Beer's law applies. A least-squares analysis of the data using standard spectra can then be utilized. Special emphasis is given to improving the response from a wavelength-modulated derivative uv-absorption spectrometer. Simulated examples are provided showing the improved analytical capabilities using the procedure outlined.

© 1978 Optical Society of America

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