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Effects of multiple-passage probabilities on fluorescent signals from biological media

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Abstract

By applying a random-walk model for photon migration, we find an exact expression for fluorescent signals emitted from a homogeneous, optically turbid medium containing a single fluorescent mass. In contrast to diffusion-theory-based models, our analysis accounts for multiple photon passages through the fluorophore sites and allows for a variable degree of fluorescent absorptivity. We particularly discuss effects on the amplitudes and phase shifts of frequency-domain fluorescent signals.

© 1997 Optical Society of America

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