Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Multiple-fiber probe design for fluorescence spectroscopy in tissue

Not Accessible

Your library or personal account may give you access

Abstract

The fiber-optic probe is an essential component of many quantitative fluorescence spectroscopy systems, enabling delivery of excitation light and collection of remitted fluorescence in a wide variety of clinical and laboratory situations. However, there is little information available on the role of illumination-collection geometry to guide the design of these components. Therefore we used a Monte Carlo model to investigate the effect of multifiber probe design parameters—numerical aperture, fiber diameter, source-collection fiber separation distance, and fiber-tissue spacer thickness—on light propagation and the origin of detected fluorescence. An excitation wavelength of 400 nm and an emission wavelength of 630 nm were simulated. Noteworthy effects included an increase in axial selectivity with decreasing fiber size and a transition with increasing fiber-tissue spacer size from a subsurface peak in fluorophore sensitivity to a nearly monotonic decrease typical of single-fiber probes. We provide theoretical evidence that probe design strongly affects tissue interrogation. Therefore application-specific customization of probe design may lead to improvements in the efficacy of fluorescence-based diagnostic devices.

© 2002 Optical Society of America

Full Article  |  PDF Article
More Like This
Effects of fiber-optic probe design and probe-to-target distance on diffuse reflectance measurements of turbid media: an experimental and computational study at 337 nm

Thanassis Papaioannou, Norris W. Preyer, Qiyin Fang, Adam Brightwell, Michael Carnohan, Greg Cottone, Russel Ross, Linda R. Jones, and Laura Marcu
Appl. Opt. 43(14) 2846-2860 (2004)

Selective detection of fluorophore layers in turbid media: the role of fiber-optic probe design

T. Joshua Pfefer, L. Stephanie Matchette, Amanda M. Ross, and Marwood N. Ediger
Opt. Lett. 28(2) 120-122 (2003)

Half-ball lens couples a beveled fiber probe for depth-resolved spectroscopy: Monte Carlo simulations

Franck Jaillon, Wei Zheng, and Zhiwei Huang
Appl. Opt. 47(17) 3152-3157 (2008)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (9)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved