Abstract

There are two goals in this simulation study: (1) to show that the time variation of the bioluminescence source can cause artifacts in the tomographic images such that quantification and localization becomes impossible; and (2) to show that the a priori knowledge of the light kinetics can be used to eliminate these artifacts. These goals are motivated by the fact that the half-life of luciferase has been reported as 30 min to 2 h in vivo. We perform two-dimensional simulations. We consider a $40\text{\hspace{0.17em} mm}$ diameter circular region with an inclusion of $6\text{\hspace{0.17em} mm}$ diameter located $10\text{\hspace{0.17em} mm}$ away from the center. The measurement data is simulated using a finite-element-based forward solver. We model the noncontact measurements such that four-wavelength data is collected from four 90° apart views. The results show that the ratio of the total imaging time to the half-life of the exponentially decaying bioluminescent source is the deciding factor in the reconstruction of the source. It is also demonstrated that a priori knowledge of the source kinetics is required to perform tomographic bioluminescence imaging of short half-life bioluminescent sources and the use of spatial a priori information alone is not adequate.

© 2008 Optical Society of America

Validation of luminescent source reconstruction using single-view spectrally resolved bioluminescence images

John Virostko, Alvin C. Powers, and E. Duco Jansen
Appl. Opt. 46(13) 2540-2547 (2007)

Tomographic bioluminescence imaging with varying boundary conditions

Vadim Y. Soloviev
Appl. Opt. 46(14) 2778-2784 (2007)

Two-dimensional bioluminescence tomography: numerical simulations and phantom experiments

Senhu Li, Qizhi Zhang, and Huabei Jiang
Appl. Opt. 45(14) 3390-3394 (2006)

An optimal permissible source region strategy for multispectral bioluminescence tomography

Jinchao Feng, Kebin Jia, Guorui Yan, Shouping Zhu, Chenghu Qin, Yujie Lv, and Jie Tian
Opt. Express 16(20) 15640-15654 (2008)

Practical reconstruction method for bioluminescence tomography

Wenxiang Cong, Ge Wang, Durairaj Kumar, Yi Liu, Ming Jiang, Lihong V. Wang, Eric A. Hoffman, Geoffrey McLennan, Paul B. McCray, Joseph Zabner, and Alexander Cong
Opt. Express 13(18) 6756-6771 (2005)