Abstract
The design and characterization of a simple and robust hyperspectral Raman line imaging illumination system with the use of a Powell lens is reported. The generated line uniformity is +/- 5% of total intensity with a laser power density of 12 mW/ mu m2 at the sample with a 50X/0.8 NA (numerical aperture) objective. Similar results were obtained by using other objectives. Linewidths remained near the diffraction limit for all objectives tested. Significant decreases in image acquisition time are also reported with the use of a Powell lens-illuminated hyperspectral Raman line imaging microscope equipped with an intensified charge-coupled device (CCD) detector. Hyperspectral images (100 X 350 pixels) were acquired in as little as 8 with a corresponding signal-to-noise ratio of 24.
PDF Article
More Like This
High-throughput line-illumination Raman microscopy with multislit detection
Kentaro Mochizuki, Yasuaki Kumamoto, Shunsuke Maeda, Masato Tanuma, Atsushi Kasai, Masashi Takemura, Yoshinori Harada, Hitoshi Hashimoto, Hideo Tanaka, Nicholas Isaac Smith, and Katsumasa Fujita
Biomed. Opt. Express 14(3) 1015-1026 (2023)
Cited By
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Login to access Optica Member Subscription