Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Two-photon near- and far-field fluorescence microscopy with continuous-wave excitation

Not Accessible

Your library or personal account may give you access

Abstract

We report on scanning far- and near-field two-photon microscopy of cell nuclei stained with DAPI and bisbenzimidazole Hoechst 33342 (BBI-342) with the 647-nm laser line of a cw ArKr mixed-gas laser. Two-photon-excited fluorescence images are obtained for 50–200 mW of average power at the sample. A nearly quadratic dependence of fluorescence intensity on laser power confirmed the two-photon effect. The nonlinearity was further supported by evidence of three-dimensional sectioning in a scanning far-field microscope. We find that the cw two-photon irradiation sufficient for imaging within typically 5 s does not significantly impair cell cycling of BBI-342-labeled live cells. Finally, high-resolution imaging in scanning near-field microscopy with good contrast is demonstrated.

© 1998 Optical Society of America

Full Article  |  PDF Article
More Like This
Combined Raman and continuous-wave-excited two-photon fluorescence cell imaging

Natallia Uzunbajakava and Cees Otto
Opt. Lett. 28(21) 2073-2075 (2003)

Near-field scanning optical microscopy of single molecules by femtosecond two-photon excitation

Michael K. Lewis, Peter Wolanin, Ari Gafni, and Duncan G. Steel
Opt. Lett. 23(14) 1111-1113 (1998)

Image contrast enhancement for two-photon fluorescence microscopy in a turbid medium

Vincent Daria, Carlo Mar Blanca, Osamu Nakamura, Satoshi Kawata, and Caesar Saloma
Appl. Opt. 37(34) 7960-7967 (1998)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (1)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved