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Characteristics of fluorescence quenching by polystyrene microspheres in tissue-simulating phantoms

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Abstract

Polystyrene microspheres were previously reported to reduce fluorophore quantum yield via collisional quenching in fluorescein-based tissue-simulating phantoms. Here, we report decreases of fluorescence intensities and lifetimes of ~10-30% that are dependent on microsphere size.

© 2004 Optical Society of America

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