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Polarization sensitive three-dimensional nanoscopy with a double-helix microscope

Sri Rama Prasanna Pavani, Jennifer G. DeLuca, and Rafael Piestun

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Abstract

We demonstrate polarization sensitive detection with 3D super-localization of single-molecules and unveil 3D polarization specific characteristics of single-molecules within the intracellular structure of PtK1 cells expressing photoactivatable green fluorescent protein.

© 2010 Optical Society of America

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