Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
Conferences > CLEO > 2010 > CThCC > CThCC2

Double-helix microscopy for wide-field 3D single-molecule fluorescence imaging

Ginni Sharma, Sri Rama Prasanna Pavani, Sean Quirin, and Rafael Piestun

Not Accessible

Your library or personal account may give you access

Abstract

We present methods to improve the localization accuracy in wide-field 3D single-molecule double-helix microscopy. We analyze the optical efficiency of the system, the fundamental limit for 3D localization, the estimation algorithms, and polarization sensitive detection.

© 2010 Optical Society of America

PDF Article
More Like This
3D Super-resolution Imaging of Microtubules with a Double Helix Point Spread Function Microscope

Ginni Grover, Keith DeLuca, Sean Quirin, Jennifer DeLuca, and Rafael Piestun
BM4B.3 Biomedical Optics (BIOMED) 2012

The Double-Helix Microscope Enables Precise and Accurate Measurement of 3D Single-Molecule Orientation and Localization Beyond the Diffraction Limit

Mikael P. Backlund, Matthew D. Lew, Adam S. Backer, Steffen J. Sahl, Ginni Grover, Anurag Agrawal, Rafael Piestun, and W. E. Moerner
NM2B.2 Novel Techniques in Microscopy (NTM) 2013

Measuring the 3D Position and Orientation of Single Molecules Simultaneously and Accurately with the Double Helix Microscope

Matthew D. Lew, Mikael P. Backlund, Adam S. Backer, Steffen J. Sahl, Ginni Grover, Anurag Agrawal, Rafael Piestun, and W. E. Moerner
CTu3N.3 CLEO: Science and Innovations (CLEO:S&I) 2013

View More...
Select as filters
Select Topics Cancel
Top
       
© Copyright 2024 | Optica Publishing Group. All rights reserved, including rights for text and data mining and training of artificial technologies or similar technologies.
Privacy | Terms of Use