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Tracking of micrometer-sized objects with high-numerical aperture lensless digital holographic microscopy

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Abstract

A method for tracking micrometer sized particles with high numerical aperture lensless holographic microscopy is presented. A composed in line hologram is reconstructed for different planes parallel to the hologram. From the projection of all the reconstructed images an artificial image is generated on a plane parallel to the reconstruction planes. From the artificial image the center of mass of each group of at least eight connected pixels is computed. Those centers of mass are the origins for volumes of interest along the propagation direction. By analyzing the maximum intensity along the volumes of interest, the method uses a two features approach to search for the best focal planes and to distinguish particles from artifacts and other elements on the reconstructed images. The method is illustrated with numerically generated in line holograms and a real experiment. The method correctly recovered the number and position of the modeled particles.

© 2012 Optical Society of America

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