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Conference Paper
European Conference on Biomedical Optics
Munich Germany
June 17, 2007
ISBN: 9780819467720
FLIM AND 2-PHOTON EXCITATION (FPE)

Sensing metabolic activity in tissue engineered constructs

Malavika Chandra, Robert H. Wilson, Wen-Liang Lo, Karthik Vishwanath, Kenji Izumi, Stephen E. Feinberg, and Mary-Ann Mycek

http://dx.doi.org/10.1364/ECBO.2007.6628_10


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Abstract

Tissue engineered constructs can be employed to graft wounds or replace diseased tissue. Non-invasive methods are required to assess cellular viability in these constructs both pre- and post- implantation into patients. In this study, Monte Carlo simulations and fluorescence experiments were executed on ex vivo produced oral mucosa equivalent (EVPOME) constructs to investigate the fluorescence emitted at 355 nm excitation from these constructs. Both simulations and experiments indicated the need to investigate alternative excitation wavelengths in order to increase the cellular fluorescence from these constructs, while decreasing contributions from extra-cellular fluorophores.

© 2007 SPIE

Citation
M. Chandra, R. H. Wilson, W. Lo, K. Vishwanath, K. Izumi, S. E. Feinberg, and M. Mycek, "Sensing metabolic activity in tissue engineered constructs," in Diagnostic Optical Spectroscopy in Biomedicine IV, D. Schweitzer and M. Fitzmaurice, eds., Vol. 6628 of Proceedings of SPIE-OSA Biomedical Optics (Optical Society of America, 2007), paper 6628_10.
http://www.opticsinfobase.org/abstract.cfm?URI=ECBO-2007-6628_10


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