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Conference Paper
European Conference on Biomedical Optics
Munich Germany
June 17, 2007
ISBN: 9780819467775
UNDERSTANDING LIFE PROCESSES: INNOVATIVE ANALYSIS, DETECTION AND DIAGNOSTIC METHODS IV (ULPIV)

Autofocus algorithms for digital-holographic microscopy

Patrik Langehanenberg, Björn Kemper, and Gert von Bally

http://dx.doi.org/10.1364/ECBO.2007.6633_13


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Abstract

Digital-holographic metrology enables quantitative phase contrast microscopy of reflective and (partially) transparent samples. In this way, new application fields are opened up for nondestructive investigations of technical samples as well as for marker-free and time-resolved analysis of cell biological processes. Studies on long-term biological processes require permanent focus position readjustment to maintain an optimum image quality. Digital holographic microscopy permits subsequent numerical focusing by variation of the propagation distance. Here, the determination of the optimal propagation distance for a sharply focused image is of particular importance. At the Laboratory of Biophysics image definition quantification algorithms were adapted to the requirements of digital holographic microscopy. In order to obtain robust and reliable algorithms, the object-dependent optical absorption properties were taken into consideration. Automatic focus tracking is demonstrated on investigations with digital holographic microscopy on both technical amplitude objects and cytological pure phase objects.

© 2007 SPIE

Citation
P. Langehanenberg, B. Kemper, and G. v. Bally, "Autofocus algorithms for digital-holographic microscopy," in Biophotonics 2007: Optics in Life Science, J. Popp and G. von Bally, eds., Vol. 6633 of Proceedings of SPIE-OSA Biomedical Optics (Optical Society of America, 2007), paper 6633_13.
http://www.opticsinfobase.org/abstract.cfm?URI=ECBO-2007-6633_13


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