Detection and visualization improvement of Spermatozoa cells by Digital Holography

L. Miccio; A. Finizio; P. Memmolo; M. Paturzo; F. Merola; G. Coppola; G. Di Caprio; M. Gioffré; R. Puglisi; D. Balduzzi; A. Galli; P. Ferraro

doi:10.1364/ECBO.2011.80890C

Molecular Imaging III
Proceedings of SPIE-OSA Biomedical Optics (Optica Publishing Group, 2011),
paper 80890C
•https://doi.org/10.1364/ECBO.2011.80890C

Detection and visualization improvement of Spermatozoa cells by Digital Holography

L. Miccio, A. Finizio, P. Memmolo, M. Paturzo, F. Merola, G. Coppola, G. Di Caprio, M. Gioffré, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro

European Conference on Biomedical Optics 2011

Munich Germany
22–26 May 2011
ISBN: 9780819486868

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NOVEL TECHNIQUES FOR MOLECULAR VISUALIZATION (NTMV)

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L. Miccio, A. Finizio, P. Memmolo, M. Paturzo, F. Merola, G. Coppola, G. Di Caprio, M. Gioffré, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, "Detection and visualization improvement of Spermatozoa cells by Digital Holography," in Molecular Imaging III, C. Lin and V. Ntziachristos, eds., Vol. 8089 of Proceedings of SPIE-OSA Biomedical Optics (Optica Publishing Group, 2011), paper 80890C.

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Abstract

Numerical analysis is implemented to investigate biological sample starting from Digital Holographic (DH) recording. The aim is to improve visualization and detection of cow spermatozoa. Digital holograms are recorded in the off-axis geometry where optical setup is a Mach-Zehnder interferometer. Then holograms are numerically manipulated to retrieve, besides the usual Quantitative Phase Map (QPM), Differential Interference Contrast (DIC) visualization. Furthermore, a new approach , named digital self-referencing holography, is described it’s able to accomplish quantitative phase analysis especially useful for specimen flowing in microfluidic channels.

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