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Conferences > FiO > 2010 > PDPA > PDPA1

Total Internal Reflection Microscopy: Calibration of the Intensity-Position Relation

Giovanni Volpe, Thomas Brettschneider, Laurent Helden, and Clemens Bechinger

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Abstract

TIRM measures particle-wall interaction potentials with nanometer and femtonewton resolution. Crucial is the knowledge of the scattering intensity-position relation I(z), usually assumed a priori exponential. The experimental determination of I(z) greatly enlarges the accessible conditions.

© 2010 Optical Society of America

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