Structured Laser Scanning Two-photon Superresolution Image with Temporal Intensity Modulation

Chia-Hua Yeh; Szu-Yu Chen

doi:10.1364/FIO.2013.FW4A.4

Frontiers in Optics 2013
OSA Technical Digest (online) (Optica Publishing Group, 2013),
paper FW4A.4
•https://doi.org/10.1364/FIO.2013.FW4A.4

Structured Laser Scanning Two-photon Superresolution Image with Temporal Intensity Modulation

Chia-Hua Yeh and Szu-Yu Chen

Frontiers in Optics 2013

Orlando, Florida United States
6–10 October 2013
ISBN: 978-1-55752-987-9

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Experimental Methods for Tissue Imaging and Therapy (FW4A)

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C. Yeh and S. Chen, "Structured Laser Scanning Two-photon Superresolution Image with Temporal Intensity Modulation," in Frontiers in Optics 2013, I. Kang, D. Reitze, N. Alic, and D. Hagan, eds., OSA Technical Digest (online) (Optica Publishing Group, 2013), paper FW4A.4.

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Abstract

To improve the lateral resolution of two-photon fluorescence microscopy (TPFM), the concept of structured illumination was applied by temporally modulating the excitation intensity. In this research, a 1.66-fold resolution improvement was experimentally demonstrated.

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