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Conference Paper
Novel Techniques in Microscopy
Vancouver Canada
April 26-30, 2009
ISBN: 978-1-55752-871-1
Superresolution I (NMA)

Three-Dimensional Super-Resolution Imaging with a Double-Helix Microscope

Sri Rama Prasanna Pavani, Rafael Piestun, Michael A. Thompson, Julie S. Biteen, and W E. Moerner


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We demonstrate three-dimensional single-molecule fluorescence imaging beyond the diffraction-limit with a double-helix point spread function microscope. Two molecules seperated 14nm (X), 26nm (Y), and 21nm (Z) are resolved within a field of view of 20x20x2?m3(XxYxZ).

© 2009 The Optical Society

OCIS Codes
(100.0100) Image processing : Image processing
(100.6640) Image processing : Superresolution
(180.0180) Microscopy : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy

S. R. P. Pavani, R. Piestun, M. A. Thompson, J. S. Biteen, and W. E. Moerner, "Three-Dimensional Super-Resolution Imaging with a Double-Helix Microscope," in Advances in Imaging, OSA Technical Digest (CD) (Optical Society of America, 2009), paper NMA5.

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