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Comparing Coherent and Spontaneous Raman Scattering Under Biological Imaging Conditions

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Abstract

We compare imaging based on coherent and spontaneous Raman scattering under conditions relevant for biological imaging. Using a broadband laser, we perform spectral domain imaging of polystyrene beads using coherent Stokes Raman scattering and spontaneous Raman scattering, finding comparable signal levels for the two methods. We present calculations to support the measurements, and discuss the implications for biological imaging.

© 2009 Optical Society of America

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