Specimen-induced aberrations frequently affect microscopes, particularly when high aperture lenses image deep into specimens. We have applied adaptive optics to correct aberrations in confocal, two-photon, widefield sectioning and harmonic generation microscopes, restoring image quality.
© 2009 The Optical Society
(010.0010) Atmospheric and oceanic optics : Atmospheric and oceanic optics
(010.1080) Atmospheric and oceanic optics : Active or adaptive optics
(180.0180) Microscopy : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy
M. J. Booth, A. Kubasik-Thayil, A. Jesacher, D. Debarre, K. Grieve, and T. Wilson, "Adaptive Optics in Biomedical Microscopy," in Advances in Imaging, OSA Technical Digest (CD) (Optical Society of America, 2009), paper NWA1.