Advances in Super-resolution Biplane FPALM, STED and 3D Particle Tracking Microscopy

Joerg Bewersdorf

doi:10.1364/NTM.2011.NMA1

Optics in the Life Sciences
OSA Technical Digest (CD) (Optica Publishing Group, 2011),
paper NMA1
•https://doi.org/10.1364/NTM.2011.NMA1

Advances in Super-resolution Biplane FPALM, STED and 3D Particle Tracking Microscopy

Joerg Bewersdorf

Novel Techniques in Microscopy 2011

Monterey, California United States
4–6 April 2011
ISBN: 978-1-55752-909-1

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Superresolution I (NMA)

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J. Bewersdorf, "Advances in Super-resolution Biplane FPALM, STED and 3D Particle Tracking Microscopy," in Optics in the Life Sciences, OSA Technical Digest (CD) (Optica Publishing Group, 2011), paper NMA1.

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Abstract

STED and FPALM microscopy generate super-resolution images at ~25 nm resolution through targeted and stochastic switching of fluorophores. I present recent advances in both techniques and introduce a novel ultra-fast 3D particle-tracking microscope.

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