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Conference Paper
Novel Techniques in Microscopy
Monterey, California United States
April 4-6, 2011
ISBN: 978-1-55752-909-1
Superresolution II (NMB)

Three-Dimensional Super-Resolution Imaging with a Corkscrew Point Spread Function

Matthew D. Lew, Steven F. Lee, and W. E. Moerner  »View Author Affiliations


http://dx.doi.org/10.1364/NTM.2011.NMB2


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Abstract

We describe the design of a corkscrew point spread function for 3D super-resolution microscopy. To prove the principle, we image fluorescent beads on a patterned PDMS surface, achieving a localization precision of 3 nm in x, 2 nm in y, and 6 nm in z.

© 2011 OSA

OCIS Codes
(110.4850) Imaging systems : Optical transfer functions
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

Citation
M. D. Lew, S. F. Lee, and W. E. Moerner, "Three-Dimensional Super-Resolution Imaging with a Corkscrew Point Spread Function," in Optics in the Life Sciences, OSA Technical Digest (CD) (Optical Society of America, 2011), paper NMB2.
http://www.opticsinfobase.org/abstract.cfm?URI=NTM-2011-NMB2


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