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Conference Paper
Novel Techniques in Microscopy
Monterey, California United States
April 4-6, 2011
ISBN: 978-1-55752-909-1
Superresolution II (NMB)

Double-Helix PSF Microscopy with a Phase Mask for Efficient Photon Collection

Sean Quirin, Ginni Grover, Callie Fiedler, and Rafael Piestun  »View Author Affiliations


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We present the first implementation of double-helix phase masks for 3D microscopy with high photon collection efficiency. The mask is fabricated using gray-level mask-less lithography. The system demonstrates precise 3D tracking of quantum dots.

© 2011 OSA

OCIS Codes
(110.4850) Imaging systems : Optical transfer functions
(180.6900) Microscopy : Three-dimensional microscopy
(220.4000) Optical design and fabrication : Microstructure fabrication

S. Quirin, G. Grover, C. Fiedler, and R. Piestun, "Double-Helix PSF Microscopy with a Phase Mask for Efficient Photon Collection," in Optics in the Life Sciences, OSA Technical Digest (CD) (Optical Society of America, 2011), paper NMB3.

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