Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
Journal Home About Issues in Progress Current Issue All Issues Feature Issues

In vivo mouse studies with bioluminescence tomography

Ge Wang, Wenxiang Cong, Kumar Durairaj, Xin Qian, Haiou Shen, Patrick Sinn, Eric Hoffman, Geoffrey McLennan, and Michael Henry

Open Access Open Access

Abstract

Bioluminescence tomography (BLT) is a new molecular imaging mode, which is being actively developed to reveal molecular and cellular signatures as labeled by bioluminescent probes in a living small animal. This technology can help diagnose diseases, evaluate therapies, and facilitate drug development with mouse models. In this paper, we describe in vivo mouse experiments with BLT, and propose the reconstruction procedure of bioluminescent sources from optical data measured on the body surface of the mouse using a modality fusion approach. The results show the feasibility of our methodology for localization and quantification of the bioluminescent activities in vivo.

©2006 Optical Society of America

Full Article  |  PDF Article
More Like This
In vivo quantitative bioluminescence tomography using heterogeneous and homogeneous mouse models

Junting Liu, Yabin Wang, Xiaochao Qu, Xiangsi Li, Xiaopeng Ma, Runqiang Han, Zhenhua Hu, Xueli Chen, Dongdong Sun, Rongqing Zhang, Duofang Chen, Dan Chen, Xiaoyuan Chen, Jimin Liang, Feng Cao, and Jie Tian
Opt. Express 18(12) 13102-13113 (2010)

Practical reconstruction method for bioluminescence tomography

Wenxiang Cong, Ge Wang, Durairaj Kumar, Yi Liu, Ming Jiang, Lihong V. Wang, Eric A. Hoffman, Geoffrey McLennan, Paul B. McCray, Joseph Zabner, and Alexander Cong
Opt. Express 13(18) 6756-6771 (2005)

Temperature-modulated bioluminescence tomography

Ge Wang, Haiou Shen, Wenxiang Cong, Shan Zhao, and Guo Wei Wei
Opt. Express 14(17) 7852-7871 (2006)

View More...

Cited By

Optica participates in Crossref's Cited-By Linking service. Citing articles from Optica Publishing Group journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (6)
Fig. 1.
Fig. 1. Our first BLT system consisting of a mouse stage, a CCD camera, a mouse holder and so on.

Download Full Size | PDF

Fig. 2
Fig. 2 Luminescent views (in pseudo color) of the side surface of the first mouse body taken by the CCD camera from four directions of 90 degrees apart. The luminescent views are superimposed on the corresponding mouse photographs. (a)–(d) Anterior-posterior, right lateral, posterior-anterior, left lateral views, respectively (the dotted pink line and the small yellow dot in (a) represent the path of the catheter and the luminescent liquid, respectively)

Download Full Size | PDF

Fig. 3.
Fig. 3. First mouse model and associated bioluminescent measurement. (a) A geometrical model of the mouse chest consisting of muscle, a heart, a lung and a liver, and (b) measured bioluminescent data mapped onto the finite element mesh model of the mouse chest.

Download Full Size | PDF

Fig. 4.
Fig. 4. BLT reconstruction of the luminescent source distribution in the first mouse. (a) A 2D transverse section of the reconstructed light source (red) and contours representing boundaries of various organs (blue), (b) the true source (inside the black circle) in a micro-CT slice superimposed with the reconstructed source (red) (the enlarged bronchus airway on the left is due to the inserted luminescent tube), and (c) a 3D rendering of the reconstructed source distribution (inside the red ellipse) in the mouse.

Download Full Size | PDF

Fig. 5.
Fig. 5. Four bioluminescent views in pseudo-color superimposed on the corresponding photographs of the second mouse. (a)-(d) Anterior-posterior, right lateral, posterior-anterior and left lateral views, respectively.

Download Full Size | PDF

Fig. 6.
Fig. 6. BLT reconstruction and histological verification. (a) Two bioluminescent sources reconstructed on the two kidneys respectively, and (b) two tumors at the same locations on the dissected kidneys.

Download Full Size | PDF

Tables (1)

Tables Icon

Table 1. Optical parameters for the mouse organ regions

View Table

Equations (4)

Equations on this page are rendered with MathJax. Learn more.

{ − ∇ ∙ ( D ( r ) ∇ Φ ( r ) ) + μ a ( r ) Φ ( r ) = S ( r ) ( r ∈ Ω ) Φ ( r ) + 2 A ( r ) D ( r ) ( v ( r ) ∙ ∇ Φ ( r ) ) = 0 ( r ∈ ∂ Ω )
Φ ˜ ( r ) = − D ( r ) ( v ∙ ∇ Φ ( r ) ) ( r ∈ ∂ Ω ) ,
AS = Φ ˜ .
min 0 ≤ S ≤ U S ∈ Ω S ∣ AS − Φ ˜ ∣ W 2 + εη ( S ) ,
Select as filters
Select Topics Cancel
Top
       
© Copyright 2024 | Optica Publishing Group. All rights reserved, including rights for text and data mining and training of artificial technologies or similar technologies.
Privacy | Terms of Use