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Three-dimensional optical force field on a Chinese hamster ovary cell in a fiber-optical dual-beam trap

Ming-Tzo Wei, Kun-Ta Yang, Artahses Karmenyan, and Arthur Chiou

Open Access Open Access

Abstract

We used a fiber-optical dual-beam trap (single-mode fiber, λ = 532nm, trapping power ∼ 22mW, the distance between the two fiber end-faces = 125μm) to capture a Chinese hamster ovary (CHO) cell with a diameter of approximately 15μm and tracked its three-dimensional Brownian motion via a pair of orthogonal quadrant photodiodes. By analyzing the Brownian motion of the trapped CHO cell, we determined the force constants of the optical force field on the CHO cell to be kx=6.75 pN/μm, ky=5.53 pN/μm, kz=1.96 pN/μm, and kx=2.91 pN/μm, ky=2.7 pN/μm, kz=0.79 pN/μm, respectively, before and after the CHO cell was treated with latrunculin, a toxic drug known to disrupt the cytoskeleton of the cell.

©2006 Optical Society of America

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Figures (6)
Fig. 1.
Fig. 1. A schematic diagram of our experimental setup

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Fig. 2.
Fig. 2. (a) A 2.58 μm silica particle trapped in a fiber-optical dual-beam trap, (b) the silica particle driven to the right by a single beam after the beam on the right hand side was turned off.

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Fig. 3.
Fig. 3. Calibration for the conversion of the QPD output voltage to the particle displacement on the optical axis via incoherent imaging on a CCD camera.

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Fig. 4.
Fig. 4. Calibration for the conversion of the QPD output voltage to the particle displacement via the power spectrum of the particle thermal fluctuation and the theoretical fitting (the red line) to a Lorentzian form.

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Fig. 5.
Fig. 5. Experimental data representing the parabolic optical force fields E(x) “□” (in black) and E(z) “□” (in blue) on a 2.58μm silica particle. The solid lines represent the theoretical fits.

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Fig. 6.
Fig. 6. CHO cell trapped and stretched in a fiber-optical dual-beam trap. Total trapping (and stretching) optical power: (a) 22mW, (b) 27mW, (c) 38mW, (d) 50mW.

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Tables (1)

Tables Icon

Table. 1. A comparison of optical force constants for silica particles of different sizes and for CHO cell with and without latrunculin treatment.

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Equations (3)

Equations on this page are rendered with MathJax. Learn more.

S v ( f ) = K B T / β 2 6 π 3 η r ( f c 2 + f 2 )
ρ ( z ) = C exp [ − E ( z ) / K B T ]
E ( z ) = − K B T ln ρ ( z ) + K B T ln C = k z Z 2 / 2
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