We integrate a holographic optical tweezer system with a double-helix point spread function imaging for high precision three-dimensional (3D) multi-particle tracking. We perform precise quantitative estimates of the 3D forces in an optical trap.
© 2011 OSA
D. B. Conkey, R. P. Trivedi, S. R. P. Pavani, I. I. Smalyukh, and R. Piestun, "Engineered point spread functions for 3D parallel particle tracking of optically trapped particles," in Optics in the Life Sciences, OSA Technical Digest (CD) (Optical Society of America, 2011), paper OTMD2.
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