Abstract
We report the use of phytochrome A (phyA), a plant protein that can reversibly switch between two states with different absorption maxima (at 660 and 730 nm), as a contrast agent for molecular contrast optical coherence tomography (MCOCT). Our MCOCT scheme builds up a difference image revealing the distribution of phyA within a target sample from pairs of consecutive OCT A-scans acquired at a probe wavelength of 750 nm, both with and without additional illumination of the target sample with 660-nm light. We demonstrate molecular imaging with this new MCOCT modality in a target sample containing a mixture of 0.2% Intralipid and of phyA.
© 2004 Optical Society of America
Full Article | PDF ArticleMore Like This
Changhuei Yang, Laura E. L. McGuckin, John D. Simon, Michael A. Choma, Brian E. Applegate, and Joseph A. Izatt
Opt. Lett. 29(17) 2016-2018 (2004)
Chenyang Xu, Jian Ye, Daniel L. Marks, and Stephen A. Boppart
Opt. Lett. 29(14) 1647-1649 (2004)
K. Divakar Rao, Michael A. Choma, Siavash Yazdanfar, Andrew M. Rollins, and Joseph A. Izatt
Opt. Lett. 28(5) 340-342 (2003)