Abstract

We present a novel fluorescence lifetime tomography system applied to a highly scattering autofluorescent phantom containing live cells expressing the fluorophore enhanced green fluorescent protein (EGFP). The fluorescence signal was excited using a fiber-laser-pumped supercontinuum source and detected using wide-field time gating imaging. To facilitate rapid 3D reconstruction of the fluorescence lifetime distribution, the time-resolved data were Fourier-transformed in time to give complex functions that formed a data set for the Fourier domain reconstruction. Initially the presence of an unspecified background autofluorescence signal impeded reconstruction of the lifetime distribution, but we show that this problem can be addressed using a simple iterative technique.

© 2007 Optical Society of America

In vivo fluorescence lifetime tomography of a FRET probe expressed in mouse

James McGinty, Daniel W. Stuckey, Vadim Y. Soloviev, Romain Laine, Marzena Wylezinska-Arridge, Dominic J. Wells, Simon R. Arridge, Paul M. W. French, Joseph V. Hajnal, and Alessandro Sardini
Biomed. Opt. Express 2(7) 1907-1917 (2011)

Fluorescence lifetime imaging by using time-gated data acquisition

Vadim Y. Soloviev, Khadija B. Tahir, James McGinty, Dan S. Elson, Mark A. A. Neil, Paul M. W. French, and Simon R. Arridge
Appl. Opt. 46(30) 7384-7391 (2007)

High speed optically sectioned fluorescence lifetime imaging permits study of live cell signaling events

D. M. Grant, J. McGinty, E. J. McGhee, T. D. Bunney, D. M. Owen, C. B. Talbot, W. Zhang, S. Kumar, I. Munro, P. M. P. Lanigan, G. T. Kennedy, C. Dunsby, A. I. Magee, P. Courtney, M. Katan, M. A. A. Neil, and P. M. W. French
Opt. Express 15(24) 15656-15673 (2007)

Three-dimensional imaging of Förster resonance energy transfer in heterogeneous turbid media by tomographic fluorescent lifetime imaging

James McGinty, Vadim Y. Soloviev, Khadija B. Tahir, Romain Laine, Daniel W. Stuckey, Joseph V. Hajnal, Alessandro Sardini, Paul M. W. French, and Simon R. Arridge
Opt. Lett. 34(18) 2772-2774 (2009)

In vivo fluorescence lifetime optical projection tomography

James McGinty, Harriet B. Taylor, Lingling Chen, Laurence Bugeon, Jonathan R. Lamb, Margaret J. Dallman, and Paul M. W. French
Biomed. Opt. Express 2(5) 1340-1350 (2011)