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Multiscale multimodal imaging with multiphoton microscopy and optical coherence tomography

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Abstract

A multiscale multiphoton microscopy (MPM) and optical coherence tomography (OCT) system has been developed using a sub-10fs Ti:sapphire laser. The system performs cross-sectional OCT imaging over millimeter field-of-view and en-face high-resolution MPM imaging with submicrometer resolution from the same sample location. With fish cornea, we have demonstrated cross-sectional imaging of cornea tissue layers using OCT, and the zoom-in imaging of cells and collagen fibers in each layer using MPM. The multiscale MPM/OCT system shows the potential of a rapid coarse scan to search for abnormal regions and the subsequent fine zoom-in imaging for diagnosis.

©2011 Optical Society of America

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