Abstract

A compact clinically compatible fluorescence lifetime imaging microscopy (FLIM) system was designed and built for intraoperative disease diagnosis and validated in vivo in a hamster oral carcinogenesis model. This apparatus allows for the remote image collection via a flexible imaging probe consisting of a gradient index objective lens and a fiber bundle. Tissue autofluorescence (337 nm excitation) was imaged using an intensified CCD with a gate width down to 0.2 ns. We demonstrate a significant contrast in fluorescence lifetime between tumor (1.77+/-0.26 ns) and normal (2.50+/-0.36 ns) tissues at 450 nm and an over 80% intensity decrease at 390 nm emission in tumor versus normal areas. The time-resolved images were minimally affected by tissue morphology, endogenous absorbers, and illumination. These results demonstrate the potential of FLIM as an intraoperative diagnostic technique.

© 2009 Optical Society of America

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History
Original Manuscript: February 11, 2009
Manuscript Accepted: May 15, 2009
Revised Manuscript: May 13, 2009
Published: June 30, 2009

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Author Affiliations

Daniel S. Elson

Institute of Biomedical Engineering

Yinghua Sun, Jennifer Phipps, Heather Stoy, Steven Tinling, Jeremy Meier, Brian Poirier, Frank S. Chuang, D. Gregory Farwell, Laura Marcu

University of California-Davis

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