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Quantitative in vivo monitoring of methemoglobinemia using noninvasive Diffuse Optical Spectroscopy

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Abstract

We present noninvasive in vivo measurements of methemoglobin (MetHb) formation and reduction in rabbits using diffuse optical spectroscopy (DOS). The absolute concentrations of MetHb, Hb-R, and Hb-O2 were quantified during nitrite infusions and again during methylene blue treatment. The fractions of MetHb to the total hemoglobin concentration correlate very closely with cooximetry values (R2 > 0.83).

© 2004 Optical Society of America

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