Auto-confocal microcopy using non-linear transmitted light detection

T. Pons; J. Mertz

doi:10.1364/BIO.2004.ThE3

Biomedical Topical Meeting
OSA Technical Digest (Optica Publishing Group, 2004),
paper ThE3
•https://doi.org/10.1364/BIO.2004.ThE3

Auto-confocal microcopy using non-linear transmitted light detection

T. Pons and J. Mertz

Biomedical Topical Meeting 2004

Miami Beach, Florida United States
14–17 April 2004
ISBN: 1-55752-769-5

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Instrumentation and Technology in the Neurosciences (ThE)

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T. Pons and J. Mertz, "Auto-confocal microcopy using non-linear transmitted light detection," in Biomedical Topical Meeting, OSA Technical Digest (Optica Publishing Group, 2004), paper ThE3.

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Abstract

We describe a novel transmission confocal laser scanning microscopy where a nonlinear crystal replaces the pinhole. Phase contrast imaging is evaluated theoretically and demonstrated in brain slices. This technique can be implemented with two-photon microscopy.

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