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In vivo Multiphoton Fluorescence Lifetime Imaging of Free and Protein-bound NADH in Normal and Pre-cancerous Epithelia

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Abstract

Multiphoton fluorescence lifetime imaging microscopy (FLIM) is a three-dimensional functional imaging technique with cellular resolution. Multiphoton FLIM of the endogenous metabolic coenzyme NADH was utilized for the non-invasive characterization of epithelial pre-cancers.

© 2006 Optical Society of America

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