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Conference Paper
Biomedical Topical Meeting
Fort Lauderdale, Florida United States
March 19, 2006
Poster Session III (TuI)

Fluorescence Lifetime Imaging Microscopy (FLIM) Measures Oxygen Gradients in Microfluidic Bioreactors

Dhruv Sud, Geeta Mehta, Khamir Mehta, Jennifer Linderman, Shuichi Takayama, and Mary-Ann Mycek

http://dx.doi.org/10.1364/BIO.2006.TuI52


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Abstract

A unique FLIM system was employed to quantitatively image extracellular oxygen distributions in perfused micro-bioreactors. We report effects of cell density on oxygen consumption and the first such measurement of oxygen gradients formed within micro-bioreactors.

© 2006 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(230.0230) Optical devices : Optical devices
(230.3990) Optical devices : Micro-optical devices

Citation
D. Sud, G. Mehta, K. Mehta, J. Linderman, S. Takayama, and M. Mycek, "Fluorescence Lifetime Imaging Microscopy (FLIM) Measures Oxygen Gradients in Microfluidic Bioreactors," in Biomedical Optics, Technical Digest (CD) (Optical Society of America, 2006), paper TuI52.
http://www.opticsinfobase.org/abstract.cfm?URI=BIO-2006-TuI52


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