A unique FLIM system was employed to quantitatively image extracellular oxygen distributions in perfused micro-bioreactors. We report effects of cell density on oxygen consumption and the first such measurement of oxygen gradients formed within micro-bioreactors.
© 2006 Optical Society of America
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(230.0230) Optical devices : Optical devices
(230.3990) Optical devices : Micro-optical devices
D. Sud, G. Mehta, K. Mehta, J. Linderman, S. Takayama, and M. Mycek, "Fluorescence Lifetime Imaging Microscopy (FLIM) Measures Oxygen Gradients in Microfluidic Bioreactors," in Biomedical Optics, Technical Digest (CD) (Optical Society of America, 2006), paper TuI52.
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