Analysis of a Two-PMT System for Simultaneous Back- and Forward-Fluorescence Detection in Multiphoton Microscopy

Ricardo Toledo-Crow; Songhai Shi; Yongbiao Li

doi:10.1364/BIOMED.2008.BMD56

Biomedical Optics
OSA Technical Digest (CD) (Optica Publishing Group, 2008),
paper BMD56
•https://doi.org/10.1364/BIOMED.2008.BMD56

Analysis of a Two-PMT System for Simultaneous Back- and Forward-Fluorescence Detection in Multiphoton Microscopy

Ricardo Toledo-Crow, Songhai Shi, and Yongbiao Li

Biomedical Optics 2008

St. Petersburg, Florida United States
16–19 March 2008
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BIOMED Poster Session II (BMD)

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R. Toledo-Crow, S. Shi, and Y. Li, "Analysis of a Two-PMT System for Simultaneous Back- and Forward-Fluorescence Detection in Multiphoton Microscopy," in Biomedical Optics, OSA Technical Digest (CD) (Optica Publishing Group, 2008), paper BMD56.

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Abstract

A two-PMT system is analyzed as an opportunity to improve the S/N ratio in a multiphoton microscope. Conditions under which this arrangement is optimal and sub-optimal are presented and discussed with representative data.

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