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Conference Paper
Biomedical Optics
St. Petersburg, Florida United States
March 16-19, 2008
BIOMED Poster Session I (BSuE)

A New Water-Soluble Near-Neutral Ratiometric Fluorescent pH Indicator

Sheng Yao, Katherine J. Schafer-Hales, and Kevin D. Belfield


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We report a new near-neutral pH indicator whose pKa of ~7.0 was determined by both absorption and fluorescence methods. It has a distinctive isoemissive point, good dispersion in cell cytosol, and low cytotoxicity.

© 2008 Optical Society of America

OCIS Codes
(160.0160) Materials : Materials
(160.2540) Materials : Fluorescent and luminescent materials
(180.0180) Microscopy : Microscopy
(180.4315) Microscopy : Nonlinear microscopy

S. Yao, K. J. Schafer-Hales, and K. D. Belfield, "A New Water-Soluble Near-Neutral Ratiometric Fluorescent pH Indicator," in Biomedical Optics, OSA Technical Digest (CD) (Optical Society of America, 2008), paper BSuE90.

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