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Conferences > BIOMED > 2010 > BSuD > BSuD39

Non-Negative Matrix Factorization to unmix several fluorescence spectra and remove autofluorescence from in vivo spectrally resolved acquisitions

Anne-Sophie Montcuquet, Lionel Hervé, Fabrice Navarro, Jean-Marc Dinten, and Jérôme I. Mars

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Abstract

Plurality of specific fluorescent markers in multiplexing, and autofluorescence of biological tissues limit specific fluorescence detection. A spectroscopic approach and a blind source separation method are proposed to unmix multiple fluorescence spectra and remove autofluorescence.

© 2010 Optical Society of America

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