Abstract

Non-invasive optical projection tomography (OPT) provides a method to image small animals in-vivo. We present two correction methods for this technique which can be applied after the images have been acquired. The first method allows to reconstruct specimens embedded in media with arbitrary refractive index, and the second method corrects for small movements of the specimens with respect to the detection system. We have tested the correction methods with computer simulated data and with samples of Parhyale hawaiensis embedded in sea water. These post-acquisition corrections prove to be a major step towards routine in-vivo imaging, and provide 3D analyses of OPT data which can not be rendered using standard reconstruction methods.

© 2010 Optical Society of America