Dynamic visualization of mitochondria within cardiomyocytes using third harmonic generation microscopy reveals intensity fluctuations similar to those seen in fluorescence microscopy. These changes bring a photobleaching free method for physiological investigations of mitochondria in cardiomyocytes.
© 2005 Optical Society of America
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy
(180.5810) Microscopy : Scanning microscopy
V. Barzda, C. Greenhalgh, J. Aus derAu, S. Elmore, H. van Beek, and J. Squier, "Dynamic Visualization of Mitochondria in Cardiomyocytes with Third Harmonic Generation Microscopy," in Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science and Photonic Applications Systems Technologies, Technical Digest (CD) (Optical Society of America, 2005), paper CWP5.
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