We report dual-color superresolution imaging using endogenously expressed fluorescent proteins. An imaging resolution of 20-30 nm facilitates study of the ultrastructural relationship between proteins present in adhesion complexes at the surfaces of whole, fixed cells.
© 2008 Optical Society of America
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy
(180.2520) Microscopy : Fluorescence microscopy
H. Shroff, C. G. Galbraith, J. A. Galbraith, H. White, J. Gillette, S. Olenych, M. W. Davidson, and E. Betzig, "Dual-Color Superresolution Imaging Using Genetically Expressed Probes," in Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference and Photonic Applications Systems Technologies, OSA Technical Digest (CD) (Optical Society of America, 2008), paper CFT1.