We present preliminary experimental data revealing depletion dynamics for stimulated emission depletion (STED) microscopy as a function of excitation intensity, depletion intensity, time delay, and fluorophore damage. FluoSpheres 350/440 (Invitrogen) is used.
© 2008 Optical Society of America
M. C. Chiang, J. C. Garcia, and J. Liu, "Depletion Dynamics for Stimulated Emission Depletion (STED) Microscopy," in Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference and Photonic Applications Systems Technologies, OSA Technical Digest (CD) (Optical Society of America, 2008), paper CFT4.
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