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Conference Paper
Conference on Lasers and Electro-Optics
San Jose, California United States
May 4-9, 2008
ISBN: 978-1-55752-859-9
Lab-on-a-Chip for Biophotonic Applications II (CThZ)

Effect of Fluorescently Labeling Protein Probes on Kinetics of Protein-Ligand Reactions

Yung-Shin Sun, James P. Landry, Yiyan Fei, Juntao Luo, Xiaobing Wang, Kit S. Lam, and Xiangdong Zhu

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Abstract

We study the kinetic effect of extrinsic fluorescent labeling agents on protein-ligand binding affinity and find that the kinetics is related to the loss or change of protein function when proteins are fluorescent-labeled.

© 2008 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.5810) Medical optics and biotechnology : Scanning microscopy
(240.0240) Optics at surfaces : Optics at surfaces
(240.2130) Optics at surfaces : Ellipsometry and polarimetry

Citation
Y. Sun, J. P. Landry, Y. Fei, J. Luo, X. Wang, K. S. Lam, and X. Zhu, "Effect of Fluorescently Labeling Protein Probes on Kinetics of Protein-Ligand Reactions," in Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference and Photonic Applications Systems Technologies, OSA Technical Digest (CD) (Optical Society of America, 2008), paper CThZ2.
http://www.opticsinfobase.org/abstract.cfm?URI=CLEO-2008-CThZ2


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