We study the kinetic effect of extrinsic fluorescent labeling agents on protein-ligand binding affinity and find that the kinetics is related to the loss or change of protein function when proteins are fluorescent-labeled.
© 2008 Optical Society of America
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.5810) Medical optics and biotechnology : Scanning microscopy
(240.0240) Optics at surfaces : Optics at surfaces
(240.2130) Optics at surfaces : Ellipsometry and polarimetry
Y. Sun, J. P. Landry, Y. Fei, J. Luo, X. Wang, K. S. Lam, and X. Zhu, "Effect of Fluorescently Labeling Protein Probes on Kinetics of Protein-Ligand Reactions," in Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference and Photonic Applications Systems Technologies, OSA Technical Digest (CD) (Optical Society of America, 2008), paper CThZ2.
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