We show a technique to measure the two-photon excitation spectrum of various fluorophores, based on Fourier transform nonlinear spectroscopy with the use of ultrabroadband laser (670-1100 nm).
© 2009 The Optical Society
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(300.0300) Spectroscopy : Spectroscopy
(300.6420) Spectroscopy : Spectroscopy, nonlinear
H. Hashimoto, K. Isobe, A. Suda, F. Kannari, H. Kawano, H. Mizuno, A. Miyawaki, and K. Midorikawa, "Measurement of Two-Photon Excitation Spectrum of Various Fluorophores with Fourier Transform Nonlinear Spectroscopy," in Conference on Lasers and Electro-Optics/International Quantum Electronics Conference, OSA Technical Digest (CD) (Optical Society of America, 2009), paper CME6.
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