We demonstrate the use of a double-clad fiber probe to conduct two-photon excited flow cytometry in vivo. High detection efficiency of GFP-expressing cells is demonstrated, and the initial dynamics of injected circulating cells is observed.
© 2009 The Optical Society
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(190.0190) Nonlinear optics : Nonlinear optics
(190.4180) Nonlinear optics : Multiphoton processes
Y. Chang, J. Y. Ye, T. Thomas, Z. Cao, A. E. Kotlyar, J. R. Baker Jr., and T. B. Norris, "Fiber-Optic Multiphoton in vivo Flow Cytometry," in Conference on Lasers and Electro-Optics/International Quantum Electronics Conference, OSA Technical Digest (CD) (Optical Society of America, 2009), paper CWE6.
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