Quantitative multiphoton microscopy requires knowledge of the spatio-temporal characteristics of the excitation electric field. With a unique multifocal system, we completely characterize the excitation intensity at the full numerical aperture of the excitation objective.
© 2010 The Optical Society
J. J. Field, C. G. Durfee, and J. A. Squier, "Blind FROG Pulse Characterization for Quantitative Differential Multiphoton Microscopy," in Conference on Lasers and Electro-Optics 2010, OSA Technical Digest (CD) (Optical Society of America, 2010), paper CMK2.