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Conferences > CLEO > 2010 > JWA > JWA77

Internal Quadratic Stark Effect Results in Color Hue Variations in Fluorescent Proteins with the Same Chromophore Structure

Mikhail Drobizhev, Shane Tillo, Nikolay S. Makarov, Aleksander Rebane, and Thomas Hughes

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Abstract

Genetically-encoded fluorescent proteins are widely used for bio-imaging. We employ two-photon absorption spectroscopy to show that their different hues can be explained by quadratic Stark effect due to variations of electric field within the protein.

© 2010 Optical Society of America

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