We demonstrate 3D photo-activation localization microscopy of 4μm thick samples with efficient photon collection using a phase mask fabricated by gray-level lithography. The performance limits of the system in the presence of noise are analyzed.
© 2011 OSA
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(220.4000) Optical design and fabrication : Microstructure fabrication
G. Grover, S. Quirin, and R. Piestun, "Double-Helix 3D Photo-activation Localization Microscopy with a Phase Mask for Efficient Photon Collection," in CLEO:2011 - Laser Applications to Photonic Applications, OSA Technical Digest (CD) (Optical Society of America, 2011), paper CThW4.
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