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Conference Paper
CLEO: Applications and Technology
San Jose, California United States
June 9-14, 2013
ISBN: 978-1-55752-972-5
Clinical Applications (ATh1N)

Optimization of Illumination Frequency and Preclinical Validation of a Wide-field Structured Illumination Microscope Designed for Imaging in situ Tumor Margins

Henry L. Fu, Jenna L. Mueller, Melodi Javid, David Kirsch, Nimmi Ramanujam, and J. Quincy Brown  »View Author Affiliations


http://dx.doi.org/10.1364/CLEO_AT.2013.ATh1N.3


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Abstract

We present a widefield structured illumination microscope for imaging surgical tumor margins in situ. The impact of frequency and turbidity on optical section thickness and SNR was characterized to determine the optimal imaging frequency.

© 2013 OSA

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.3890) Medical optics and biotechnology : Medical optics instrumentation

Citation
H. L. Fu, J. L. Mueller, M. Javid, D. Kirsch, N. Ramanujam, and J. Q. Brown, "Optimization of Illumination Frequency and Preclinical Validation of a Wide-field Structured Illumination Microscope Designed for Imaging in situ Tumor Margins," in CLEO: 2013, OSA Technical Digest (online) (Optical Society of America, 2013), paper ATh1N.3.
http://www.opticsinfobase.org/abstract.cfm?URI=CLEO_AT-2013-ATh1N.3


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