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Optics InfoBase > Conference Papers > CLEO_SI > 2013 > CTu3N > Page CTu3N.1 © 2013 OSA

Conference Paper
CLEO: Science and Innovations
San Jose, California United States
June 9-14, 2013
ISBN: 978-1-55752-972-5
Imaging & Microscopy II (CTu3N)

High-speed Live-cell Super-resolution Microscopy with Stochastically Switching Fluorophores

Joerg Bewersdorf  »View Author Affiliations


http://dx.doi.org/10.1364/CLEO_SI.2013.CTu3N.1


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Abstract

Super-resolution microscopy with stochastically blinking fluorophores (FPALM/PALM/STORM, etc.) achieves ~25 nm resolution by localizing molecules in thousands of camera frames. Here I present a solution to speed up recording dramatically, enabling nanoscopy at video rate.

© 2013 OSA

OCIS Codes
(100.6640) Image processing : Superresolution
(170.0180) Medical optics and biotechnology : Microscopy
(180.0180) Microscopy : Microscopy

Citation
J. Bewersdorf, "High-speed Live-cell Super-resolution Microscopy with Stochastically Switching Fluorophores," in CLEO: 2013, OSA Technical Digest (online) (Optical Society of America, 2013), paper CTu3N.1.
http://www.opticsinfobase.org/abstract.cfm?URI=CLEO_SI-2013-CTu3N.1


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