We present a double-helix point spread function (DH-PSF) optimized for particle superlocalization in three-dimensions. The DH-PSF has the lowest Cramer-Rao bound for axial estimation. The limitations to the rotation rate are investigated.
© 2009 OSA
G. Sharma, S. R. P. Pavani, and R. Piestun, "Optimization of Double-Helix Point Spread Function for Photon-Limited 3-D Imaging Systems," in Frontiers in Optics 2009/Laser Science XXV/Fall 2009 OSA Optics & Photonics Technical Digest, OSA Technical Digest (CD) (Optical Society of America, 2009), paper CTuD2.
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